Concentration (ppm)

Concentration difference

No. of alive larvae

No. of dead larvae

Mean death

 Mean death Concentration difference

00 (control)

00 (check)

100

200

300

400

500

0

0

100

100

100

100

100

20

20

19

15

12

9

0

0

0

1

5

8

11

20

0

0

0.5

3

6.5

9.5

11,5

0

0

50

300

650

950

1150

 Total 3100


The LC50 value of the seed extract of Annona squamosa for 48 h has been determined according to the arithmetic method of Karber (1931). The calculation was done as following:


LC50 = 500 – 155
LC50 = 345ppm
Table 4: LC50 value of the seed extract of Annona squamosa for 48 h.