N-source Parameter
Totalcount ×109 Total Sporex109 Protein (µg/ml) LC50 (ng/ml) Final Ph
Strain EMCC1931
(NH4)2SO4 1.40 1.3 0 0 6.6
NaNO3 1.90 1.80 0 0 6.5
NH4Cl 2.30 1.99 0 0 6.4
Beef extract 3.97 2.80 653 4.2 8.5
Peptone 2.56 2.30 448 30 8.5
Yeast extract 2.78 2.5 499 15 8.3
Beef / peptone 2.70 2.40 467 22 7.9
Beef / yeast extract 2.81 2.6 508 10 7.9
Peptone / yeast extract 2.17 2.13 340 36 8.3
Control 2.92 2.69 630 5.0 8.5
Strain EMCC1932
(NH4)2SO4 1.40 1.32 0 0 7.0
NaNO3 1.80 1.70 0 0 7.2
NH4Cl 2.70 2.50 0 0 6.9
Beef extract 3.92 3.81 600 5.0 8.6
Peptone 2.42 2.24 332 33 8.5
Yeast extract 2.57 2.6 438 25 8.3
Beef / peptone 2.43 2.46 392 29 8.4
Beef / yeast extract 2.88 2.79 452 21 9.0
Peptone / yeast extract 1.95 1.79 298 45 8.9
Strain 1593
(NH4)2SO4 2.50 2.30 0 0 6.9
NaNO3 3.31 3.20 0 0 6.8
NH4Cl 3.70 3.60 0 0 6.4
Beef extract 3.62 3.40 576 10 8.6
Peptone 2.02 2.00 427 34 8.5
Yeast extract 2.50 2.30 439 21 8.3
Beef / peptone 2.15 2.71 438 26 8.1
Beef / yeast extract 2.80 2.10 510 17 9.0
Peptone / yeast extract 1.69 1.67 276 49 8.9
Control 2.90 2.80 540 13 8.6
Strain 2297
(NH4)2SO4 2.1 1.9 0 0 6.9
NaNO3 2.5 2.3 0 0 6.8
NH4Cl 2.7 2.6 0 0 6.9
Beef extract 2.8 2.7 588 9.2 8.6
Peptone 2.4 2.2 394 39 8.5
Yeast extract 2.5 2.4 410 30 8.2
Beef / peptone 2.4 2.3 401 35 8.2
Beef / yeast extract 2.7 2.61 447 24 8.8
Peptone / yeast extract 1.87 1.85 315 42 8.2
Control 2.79 2.69 570 11 8.6
LSD0.01   2.18 1.89    
Table 6: Effect of nitrogen sources on the growth, sporulation, protein yields and mosquitocidal activities of local and reference strains of B. sphaericus.