Amplicon

Length

Mutation analysis

Mutated alleles detected, %

1

303

IVS1-110, cd39

65.5

2

459

IVS1-1, IVS1-6, IVS2-1

25.7

3

259

IVS2-745

5.2

4

255

-87, cd6, cd8

2.7
Table1: Percentage of mutant β-thalassemia alleles that can be detected by each amplicon [17].