Figure 3: Characterization of AuNP-DNA conjugate. (a) UV-Vis spectra of AuNP-DNA showing λmax=255 nm for DNA and 519 nm for AuNP (dashed line) and AuNP after heating at 60oC showing a broad peak at 520-600 nm, indicating flocculation (solid line). (b) UV-Vis spectra of the AuNP-DNA compared (dashed line) to that of the supernatant (solid line) after bioconjugation. The λmax corresponding to the DNA shifted from 260 nm (unbound) to 255 nm (bound) when conjugated to AuNP. The AuNP peak at ~520 nm is not visible in this graph because of the scale of the absorbance used.