Figure 2: Chemical characterization of the RAD54 SERS sensor. Anti-RAD54 MAbs conjugated to AgNPs via MMT linker (RAD54 SERS sensor) shows the weak peaks characteristic to AgNPs (a) and MMT linker (b) and strong peaks associated with protein or MAbs (c). The solid and the dotted arrows represent decrease in characteristic peaks of AgNPs (NO3-) and the MMT linker (Lk), respectively. Protein bands are assigned to aromatic amino acids (W: Tryptophan, Y: Tyrosine and F: Phenylalanine) and amide bonds (I: C=O, II: C-NO, III:C-NIn where O is out of phase and In for inphase). The inset TEM image (scale bar: 0.2 µm) of the RAD54 SERS sensor shows the MAbs conjugated to AgNPs.