Figure 3: Selectivity (left) and toxicity (right) of the RAD54 SERS sensor. (Left) RAD54 protein in a mixture with R-6-G detected using the RAD54 sensor (top spectra) do not show the characteristic distinct peak of R-6-G at 1503 cm-1 (bottom spectra) but only the RAD54 distinct peak at 1390 cm-1 (middle spectra). (Right) The SERS sensor has less anti-microbial activity than the bare SERS substrate and ionic silver (Ag+). The pure BY4742 S.c. is more sensitive to growth inhibitory effect of Ag than to the mixed S.c. culture. The optical density (O.D.) of the inoculum was 0.15. The error bars represent the S.D. of n=3x2 (three independent experiments done in duplicate).