Coded values of variables Factor Name Unit -2 -1 0 1 2 A Time hour 0.5 3 5 8 10.5 B Enzyme Amount % (w/w) 5 10 15 20 25 C Temperature °C 20 30 40 50 60 D Shaking Speed rpm 50 100 150 200 250 aStudy type, response surface; No. of experiments, 30; design, CCRD; response, Y1, name, flavour ester, unit, % conversion.

Table 2: Design matrix of the actual experiments carried out for developing the model.