Figure 4: The effect of cryopreservation temperature on cultured human adipose-derived cells. A) Tissues were cryopreserved at −20°C, −70°C , or −180°C in 10% DMSO + 90% FBS. B) Tissues were cryopreserved at −20°C, −70°C, or −180°C in 10% DMSO + 90% human plasma. Cells were isolated by collagenase treatment and cultured in media (DMEM containing 10% FBS) for 7 days. Scale bar = 40×, 100 μm.