Run
Yeast extract (g/L)
Glucose
(g/L)
(NH
4
)
2
SO
4
(g/L)
MgSO
4
×7H
2
O (g/L)
L-ornithine concentration（g/L）
1
20
100
50
2.5
10.59
2
18
90
45
2.3
11.24
3
16
80
40
2.1
13.33
4
14
70
35
1.9
13.08
5
12
60
30
1.7
12.98
6
10
50
25
1.5
9.13
7
8
40
20
1.3
9.52
Table 7:
Steepest ascent experiment design and experimental result
