C. Protein(mg) Lipase Activity Specific Activity F. Fold Yield % Supernatant    (crude extract) 82.46                  41.6 U/ml           46.34 U/mg     (NH4)2SO4 40% 53.7                    21.5 U/ml         25.65 U/mg 0.55         51.68 (NH4)2SO4 60% 42.6                    9.8 U/ml         17.7 U/mg 0.69         45.58 DEAE cellulose 4.54                   6.42 U/ml     204.12 U/mg 11.53         65.51

Table 2: Purification of lipase from Pseudomonas sp.