(1)Blank: 20-25ml Fresh sample of soft drink. (2)Test 1: 20-25ml Sample was incubated at room temperature without the mixture of prepared nanoparticles of emulsified encapsulated bovine serum albumin of bound glucose oxidase (2g/ml) and chymotrypsin solution (18U/ml). (3)Test 2: 20-25ml sample was incubated at room temperature with the mixture of prepared nanoparticles of emulsified encapsulated bovine serum albumin of bound glucose oxidase (2g/ml) and chymotrypsin solution (18U/ml).

Figure 5: Effect of prepared emulsified encapsulated nanoparticles of bovine serum albumin of bound glucose oxidase in the sample of (a) Coca-cola (b) Sprite (c) Fanta and (d) Appy fizz (non-alcoholic beverages).