Figure 2: IPX-750 is found in significant concentration in the blood of rats after gavage administration at 200mg/kg. Rats were administered IPX-750 at 200mg/kg by gavage, blood samples were collected at 45, 60, and 90 min post administration and separated into cell (a) and plasma (b) fractions by centrifugation at 10K for 10 min. Samples were lyophilized and IPX-750 in the lyophilized fractions was extracted into 100μl TMS derivatization reagent, PAGP internal standard was added and 1μl was injected for GC/MS analysis. GC peaks containing signature IPX-750 mass ions, or PAGP mass ions, were digitally selected and their integrated peak areas determined and used for calculation of IPX-750 concentration as described in the Methods section. The levels of IPX-750 were compared between IPX-750 injected and saline injected rats using ANOVA. n=3; all showed significance; p ≤ 0.001.