Figure 3: IPX-750 is detected in both the cell and plasma fractions after intraperitoneal (a) and gavage (b) administration. In order to assess the relative distribution of IPX-750 in the cell with respect to the plasma portion of the blood of rats, rats were administered IPX-750 at 200 mg/kg by i.p. and gavage; blood samples were collected at 30, 60, and 90 min or 45, 60, and 90 min post i.p. and gavage administration, respectively. GC peaks containing signature IPX-750 mass ions, or PAGP mass ions, were digitally selected and their integrated peak areas determined and used for calculation of IPX-750 concentration as described in the Methods section. The ratio is calculated by dividing IPX-750 levels in cells by levels in plasma. The ratios were compared between IPX-750 injected and saline injected rats using ANOVA n=3; all showed significance; p ≤ 0.001.