Figure 4: IPX-750 is detected in brain and liver tissues after administration at 200 mg/kg. The level of IPX-750 in the brain and liver were determined after intraperitoneal IPX-750 administration at 200 mg/kg either by intraperitoneal (brain) or gavage (brain and liver) administration at 30, 60 and 90 min or at 45, 60, and 90 min after intraperitoneal and gavage administration, respectively. GC peaks containing signature IPX-750 mass ions, or PAGP mass ions, were digitally selected and their integrated peak areas determined and used for calculation of IPX-750 concentration and expressed at ng IPX-750/mg of brain tissue or brain and liver tissues after intreperitoneal (a) and gavage (b) administration, respectively. The levels of IPX-750 in tissues were compared between IPX-750 injected and saline injected rats using ANOVA n=3; all showed significance; p ≤ 0.001.