Figure 2: The sequence of a siRNA molecular beacon against mce 4A. A molecular beaconwas generated against mce4 mRNA from the nonvirulent Mycobacterium, M. smegmatis MC2155 (Ms, A). This mce4 siRNA was generated from the Ms genome which spans nucleotides 5960740- 5960721 (B) which is homologous to a region in the M. tuberculosis H37Rv genome spanning nucleotides 101049-101030 (C). The fluorophore TYE 665 was conjugated to the 5’ end and the quencher, Iowa Black RQ-SP, was conjugated to the 3’ end. A random molecular beacon was also generated as a negative control that does not have any match in the entire mycobacterial or human genome (D).