Figure 5: Northern blotting analysis of DD3-3 (A), DD7-1 (B), and DD8-14 (C). Northern blotting analysis was conducted using each cDNA probe labeled with DIG. Total RNA from wild-type AX2 cells developed for 0 h (0), 3 h (3), 6 h (6), 9 h (9), and 12 h (12) was applied to each lane. The membranes were incubated with each DIG-labeled cDNA probe in upper panel. In each lower panel, aga- rose gels were stained with EtBr to confirm that the same amount of total RNA was applied to each lane.