A: RT-PCR product obtained after 35 cycles using PCR primers for PARP-1 (upper panel) and after 28 cycles for beta actin (lower panel); lane 1, negative control (PCR without cDNA), lanes 2, 3 are PCR products obtained using cDNA prepared from K-vector and untreated control K562 cells. Lanes 4, 5 represent the products obtained from KsiI cells. 32 B: RT-PCR product at different cycles using PCR primers for PARP-1 (upper panel) and beta actin (lower panel); lane 1, negative control (PCR without RT product), lanes 2, 4 are product from K-vector cells at 33 and 35 cycles respectively. Lanes 3, 5 represent the product from KsiI cells at the same PCR cycles respectively. C: Caspase-3 activity at each dose in KsiI was compared with that in K-vector and p-values were calculated as denoted by ‘#’ (0.01<p ≤ 0.05) in the figure. This experiment was repeated three times.
