Figure 8: Increase in cellular NAD+ by benzamide treatment in K562 cells. The total cell extract was prepared for NAD+ assay from untreated control cells as well as cells treated with 5 mM benzamide for different times. Time dependent increase of NAD+ pool in K562 cells. Each data points represent average of 3 independent experiments with standard deviations. p-values were calculated as denoted by ‘***’ (0.001<p≤0.01 ) in the figure.3.