Figure 2: Characterization of BMMSC grown in Chitosan scaffold in-vitro A: Successful growth and adherence of seeded cells (6.104 /5mm3) to Chitosan was assessed by nuclear stain of the sponge at day 4. Arrows indicate the nucleii of adherent BMMSC. B: Increased ALP activity of dexamethasone treated Chitosan seeded with BMMSC expressed as U/ml/hour/µg of proteins. Hek 293 cells were used as positive control. There was a statistically significant increase ALP activity * of treated versus non treated sponges seeded with BMMSC.