Figure 2: Nutlin reduces DNA damage and keratinocyte apoptosis in UVBirradiated skin. Mice (n=10) were treated on the counterlateral halves of dorsal skin with nutlin (B, D) or the isopropanol vehicle (A, C) and irradiated with 100 mJ/cm2 UVB. Skin samples taken 24 h after irradiation were stained with antithymine dimer or with TUNEL technique (see Methods). Arrows show CPD- or TUNEL-positive nuclei. Magnification x400.