Figure 4: Western blot results of the 14-3-3σ knock-down. WT-untreated control; Lipo-Lipofectamine control; siRNA-siRNA-knock-down. Western blot demonstrated the knock-down of 14-3-3σ in the HaCaT cells. Parallel the expression of Rho A, Rac1/2/3, LIMK1 and phosphorylated cofilin decreased in siRNA transfected HaCaT cells, whereas cofilin was up-regulated. Furthermore, the expression of cyclin A and E were reduced significantly, but cyclin D was weakly decreased by the knock-down. Acetylated, α-tubulin, Cdc42 and maspin were unaffected by the siRNA treatment.