Figure 2: All DIF, except e. a) positive staining of blood vessels around a hair follicular unit using FITC conjugated anti-human fibrinogen (green staining; white arrow). b) Antibody to Cy5 conjugated JAM-A on dermal blood vessels (red staining; white arrows). Additional, colocalizing FITC conjugated antihuman plasminogen antibody staining is present (green-yellow staining; white arrows). c) Similar to b, but shows staining with FITC conjugated antiplasminogen alone (green staining; white arrows). d) Positive staining of blood vessels around a dermal eccrine gland duct using Cy5 conjugated anti-JAM-A (red staining; white arrows). e) H&E demonstrating the dermal inflammatory infiltrate, including eosinophils (black arrow) (40x). f) Dermal blood vessels demonstrating positive staining with FITC conjugated anti-human fibrinogen (green staining; red arrow). g), h) Dermal blood vessel perivascular areas displaying positive staining with FITC conjugated anti-human IgG3 (green staining; white arrows). i) Positive staining of blood vessels near a hair follicular unit with FITC conjugated anti-human IgG (green staining; red arrows). Note-Keratinocyte nuclei were also counterstained with DAPI in b, d, f and i (light blue staining).