| Groups |
Treatment
|
Onset time (sec) in various phases of convulsion |
| Tonic limb flexion |
Tonic extensor |
Clonus |
Stupor |
| I |
10% w/v DMSO
(5 ml/kg, i.p) Solvent Control |
11.26 ± 0.79 |
43.54 ± 1.27 |
54.28 ± 2.89 |
70.37 ± 3.29 |
| II |
Zonisamide (35 mg/kg, i.p)
Positive Control |
48.27 ± 0.85 |
62.89 ± 2.71 |
76.14 ± 4.15 |
120.13 ± 6.28 |
| III |
A 350619 (100 µM/ kg,i.p)
sGC activator |
11.18 ± 0.39 |
36.78 ± 1.93 |
45.29 ± 2.07 |
54.39 ± 2.39 |
| IV |
Methylene blue (50 mg/kg, i.p)
sGC inhibitor |
65.21 ± 2.14 |
85.13 ± 3.28 |
117.45 ± 5.37 |
134.26 ± 5.06 |
| V |
BRL 50481 (2 mg/kg, i.p)
PDE-7 inhibitor |
6.26 ± 0.67 |
28.78 ± 1.13 |
31.29 ± 2.15 |
42.09 ± 3.28 |
| VI |
A 350619 (100 µM/ kg,i.p)
+ BRL 50481 (2 mg/kg, i.p) |
10.27 ± 0.79ΔΔΔ |
21.78 ± 2.61 **, ΔΔΔ |
28.69 ±3.37 ΔΔΔ |
39.39 ± 3.81 ΔΔΔ |
| VII |
Methylene blue (50 mg/kg, i.p) + BRL 50481 (2 mg/kg, i.p) |
51.21 ± 2.12***, ΔΔΔ, ΨΨΨ |
75.11 ± 3.28***, ΔΔΔ,ΨΨΨ |
103.25 ± 5.89***,ΨΨΨ |
121.26 ± 7.08***,ΨΨΨ |
Data represented as mean ± SEM (n=6), which represents onset time of various phases of convulsion in seconds. Treatments were given 30 mins prior to maximal electroshock (150 mA for 0.2 secs). *** denotes p<0.001, compared with A-350619 received group, Δ and ΔΔΔ denotes p<0.05 and p<0.001 compared with methylene blue received group, ΨΨΨ denotes p<0.001, compared with BRL50481 received group (One-way ANOVA followed by Tukey-Kramer multiple comparisons test). |
