Figure 2: SDS-PAGE and fluorescent western blot of BPNM. Figure A demonstrates an electrophoretic pattern consistent with PNS myelin. Lane a consists of the molecular weight (M.W.) markers (in KDa) and lane b consists of 50% pure bovine CNS myelin basic protein (MBP). Lanes c-e consist of purified BPNM from 2 separate isolates (c and d/e). Based on M.W., 1 most likely represents periaxin, 2 represents P0, 3 represents PMP-22, 4 represents post-translationally modified MBP, while 5 represents P2. 6 represents proteolipid protein (not present in PNS myelin), while 7 represents the 17-18.5 kDa fractions of MBP. Figure B confirmed that the majority of the electrophoresed proteins were glycoproteins as expected of PNS myelin. Lane a consists of 50% pure bovine CNS MBP, lanes b consist of BPNM while lanes c consist of the BSA standards. The specificity of lens culinaris-FITC is demonstrated by the lack of staining of the BSA standards. P2, the fatty acid-binding basic protein is also not detected. 1 most likely represents periaxin, 2 represents P0, 3 represents PMP-22, 4 represents post-translationally modified MBP, while 5 represents partially glycosylated CNS MBP.