Protein/Marker of Inflammation Results of stimulation studies  Description Primary Antibody Irrelevant IgG Working Concentration Manufacturer Detection System
IL-1 beta A Interleukin-1 beta, produced by activated macrophage, mediates inflammatory response including cellular proliferation, differentiation, and apoptosis Mouse monoclonal anti-human IL-1 beta Mouse IgG1 1 to 200 Sigma Aldrich AEC; Sigma Aldrich
CFI A Complement factor I is a serine protease that inactivates C3b and C4b Mouse monoclonal anti-human CFI Mouse IgG1 1 to 250 Lifespan Biosciences Vector Blue; Vector Laboratories
p-Stat3 AMD membranes Stat3 is an important signalling molecule for cytokines/growth factor receptors. Here we stained for activated Stat3 (Phospho-Stat3 (Tyr705) (D3A7)).  STAT3 is part of the JAK/STAT pathway. Rabbit monoclonal anti-mouse phospho-Stat3 Rabbit IgG 1 to 100 Cell Signaling Technology Vector Blue; Vector Laboratories
CXCL-11 AGE IFN-inducible T cell α chemoattractant is a member of the C-X-C chemokine family and is expressed in IFN-γ-treated astrocytes, monocytes, keratinocytes,bronchial epithelial cells and neutrophils Rabbit polyclonal anti-human CXCL-11 Rabbit IgG 1 to 100 Santa Cruz Biotechnology, INC AEC; Sigma Aldrich
CXCL-10 Early biomarker of AMD CXCL-10 (IP-10) is a member of the C-X-C chemokine family (pro-inflammatory) Mouse monoclonal anti-human IP-10 Mouse IgG1 1 to 100 Santa Cruz Biotechnology, INC AEC; Sigma Aldrich
RSAD2 A, AGE RSAD2 is involved in antiviral defence (against Hep C, cytomegalovirus, and HIV-1) Rabbit polyclonal anti-human RSAD2 Rabbit IgG 1 to 100 Santa Cruz Biotechnology, INC Vector Blue; Vector Laboratories
Table 2: Proteins studied and antibodies used. *Antibody detects STAT3 when STAT3 is phosphorylated at tyrosine705. Do not cross react with phopho-EGFR or other phosphor-tyrosine STAT proteins.