Figure 3: Glaucoma-related stressors do not alter localization of gp130 to RGCs, astrocytes and Müller glia in central retina. Representative fluorescent micrographs of central retina in whole eye paraffin sections from young C57 (top row), aged C57 (second row), young DBA/2 (third row) and aged DBA/2 (fourth row) mice. Co-immunolabeling with gp130 (green) and cell type-specific markers (red) for RGCs (β-tubulin; A), astrocytes (GFAP; B) and Müller glia (glutamine synthetase (C) plus DAPI counterstain (blue) reveals colocalization of gp130 (yellow) with β-tubulin, GFAP and glutamine synthetase in the GCL and NFL. Scaling is consistent for all images. GS: Glutamine synthetase; ONL: Outer nuclear layer; OPL: Outer plexiform layer; INL: Inner nuclear layer, IPL: Inner plexiform layer; GCL: Ganglion cell layer; NFL: Nerve fiber layer