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Figure 8: Axonal transport in RGCs correlates with GFAP and glutamine synthetase expression in healthy retina. Left panels: Regression graphs of fluorescent intensity for GFAP (A) and glutamine synthetase (GS; B) immunolabeling as a function of cholera toxin-β (CTB) uptake/transport in the ganglion cell and nerve fiber layers of mid-central to mid-peripheral regions of whole mounted retina from young C57 mice. Data is plotted as intensity of GFAP (y-axis; A) and glutamine synthetase (y-axis; B) versus intensity of CTB (x-axis) in arbitrary units. Solid line indicates regression line based on r2 value. Dashed line indicates the 95% confidence interval. Dotted line indicates predicted values based on r2 and 95% confidence. Right panels: Representative confocal micrographs depicting relationships between CTB transport (red) and GFAP (blue; A), and GS (blue; B) immunolabeling. For all, examples of low (left) and high (right) intensities are provided. Scaling is consistent for all images. |