Figure 11: IOP-induced glaucoma alters the strength and nature of relationships between RGC axonal transport and expression of gp130, GFAP and glutamine synthetase. Left panels: Regression graphs of fluorescent intensity for gp130 immunolabeling and intensities of GFAP (A) and glutamine synthetase immunolabeling (GS; B) as well as fluorescent intensity of CTB labeling and GFAP immunolabeling (C) in the ganglion cell and nerve fiber layers of mid-central to mid-peripheral regions of wholemounted retina from aged DBA/2 mice. A,B. Data is plotted as intensity of gp130 (y-axis) versus intensity of GFAP (x-axis; A) and GS (x-axis; B) in arbitrary units. C. Data is plotted as intensity of GFAP (y-axis) versus intensity of CTB labeling (x-axis). For all, solid lines indicate regression line based on r2 value. Dashed lines indicate the 95% confidence interval. Dotted lines indicate predicted values based on r2 and 95% confidence. Right panels: Representative confocal micrographs depicting relationships between gp130 (green) and GFAP immunolabeling (blue; A), gp130 (green) and GS immunolabeling (blue; B) and CTB transport (red) and GFAP immunolabeling (blue, C). For all, examples of low (left) and high (right) intensities are provided. Scaling is consistent for all images.