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Figure 7: Protein incorporation. A) gA channel current trace in 8x8 BLM array formed across a double-sided n-hexene modified ETFE partition. The BLM array were formed in 1N HCl adjusted to pH 1 using a 50 mg/ml DPhPC/ decane bilayer forming solution with gAadded in a 1:107 ratio. B) α-hemolysin channel current trace with experimental details as in (A) except that buffer was replaced with phosphate buffered saline solution (PBS). After BLM array formation 10μl α-hemolysin was added to the cis chamber and channel activity recorded as described in the Materials and Methods section. |