Figure 7: Protein incorporation.
A) gA channel current trace in 8x8 BLM array
formed across a double-sided n-hexene modified ETFE partition. The BLM
array were formed in 1N HCl adjusted to pH 1 using a 50 mg/ml DPhPC/
decane bilayer forming solution with gAadded in a 1:107 ratio.
B) α-hemolysin
channel current trace with experimental details as in (A) except that buffer
was replaced with phosphate buffered saline solution (PBS). After BLM
array formation 10μl α-hemolysin was added to the cis chamber and channel
activity recorded as described in the Materials and Methods section.