Figure 5: Percent live RCE cells following treatment with intracellular and extracellular components of K1263 and K1263ΔPLY for 3 hours at 37°C and 5% CO2. RCE cells exposed to media alone were considered 100% viable. Following incubation, cells were treated with 2 µM calcein AM and the absorbance measured at 485/530 nm.