DPO

Cut-off titer

MAT Sensitivity (%)

95% CI

MAT Specificity*   (%)

95% CI

IgM ELISA Sensitivity (%)

95% CI

IgM ELISA Specificity* (%)

95% CI

1-10

≥1:40

19.8

13.1-28.9

79.0

67.4-87.3

31.3

22.9-41.1

96.8

89.0-99.1

≥1:80

12.5

7.3-20.6

93.5

84.6-97.5

25.0

17.4-34.5

100.0

94.2-100

≥1:160

6.3

2.9-13.0

100.0

94.2-100.0

16.7

10.5-25.4

100.0

94.2-100

 

11-20

≥1:40

95.0

86.3-98.3

71.4

59.3-81.1

96.7

88.6-99.1

93.7

84.8-97.5

≥1:80

90.0

79.9-95.3

90.5

80.7-95.6

91.7

81.9-96.4

100.0

94.2-100.0

≥1:160

81.7

70.1-89.4

100.0

94.2-100.0

85.0

73.9-91.9

100.0

94.2-100.0

 

>20

≥1:40

92.3

83.2-96.7

71.4

59.3-81.1

89.2

79.4-94.7

93.7

84.8-97.5

≥1:80

87.7

77.5-93.6

90.5

80.7-95.6

70.8

58.8-80.4

100.0

94.2-100.0

≥1:160

81.5

70.4-89.1

100.0

94.2-100.0

58.5

46.3-69.6

100.0

94.2-100.0

Bold text indicates the optimal cut-off for sensitivity and specificity based on the ROC plots with priority on a high specificity *specificity based on unknown controls
Table 2: Sensitivity and specificity and corresponding 95% confidence intervals (CI) of either MAT or IgM ELISA.