A. Sequence alignment of the 8 spacer sequences derived from this study (1H03, 2E03, 3E04, 4G03, 5B04, 6D03, 7G11, and 8D04) along those of B. garinii PBi strain (BG), B. afzelii PKo strain (BA), and B. burgdorferi B31 strain (BB) using the Clustal W2 program (http://www.ebi.ac.uk/Tools/msa/clust-alw2/). B. Phylogenetic analyses showed that 7 sequences cluster with that of B. gari-nii, 1 with B. afzelii, and whereas none with B. burgdorferi.
Figure 2: Sequence analysis of the 5S-23S rRNA intergenic spacer of Borrelia DNA amplifi ed from the patient urine samples.