Figure 2: Activity of the EhPgp1 gene promoter (-234 to -197 bp) in drug resistant trophozoites. At the top are located the R7 and R9 repeated sequences. (A) Schematic representation of the plasmids containing different deletions of the EhPgp1 gene core promoter. The numbering refers to the transcription initiation site, designated as +1. All plasmids contain +24 bp downstream from the ATG of the EhPgp1 gene, the CAT reporter gene and the 3’-flanking actin region (3’ACT). The p268Pgp1 plasmid contains the EhPgp1 gene core promoter. The pA5’A3’CAT plasmid, used as positive control, contains 480 bp fragment of the actin gene promoter. Arrows, transcription initiation site, B, BamHI; E, EcoRI; H, HindIII; K, KpnI; S, SmaI; X, XbaI; X’, XhoI. (B) CAT activity (%) of the trophozoites transfected with the corresponding plasmids. Bars, average of CAT activities ± S.D. representative of three independent experiments performed in duplicate.