Figure 3: Identification of an activator sequence in -234 to -218 bp region of the EhPgp1 gene core promoter. (A) Schematic representation of the p268Pgp1 plasmid. Arrow, transcription initiation site; S, SmaI; H, HindIII; B, BamHI; X´, XhoI. (B) Constructions containing different mutations in R7(3) and R(9) regions (brackets), used for transfecting clone C2 trophozoites. Mutations are indicated by bold underlined font. At the bottom, schematic representation of the pA5’A3’CAT plasmid, used as positive control. E, EcoRI, K, KpnI. (C) CAT activity (%) of the trophozoites transfected with the corresponding plasmids. Bars, average of CAT activities ± S.D. representative of three independent experiments performed in duplicate.