Strains

Access Number

Hemolysis activities onto 2% sheep red blood cell plate and incubated for 48 h

Surface tension+/-STD mN/m

Ionic charge of the rinsing solution

Growth at 8°C

Growth at 17°C

Growth at 8°C

Growth at 17°C

Growth at 8°C

Growth at 17°C

PfA7B

GQ334362

β ++

 β ++

27.0+/-0.0

26.7+/-0.0

anionic

anionic

Pf1 Vis-

-

-

71.2+/-0.1

60.9+/-0.7

non ionic

non ionic

CTS38

GQ334368

 β +

 β +

63.8+/-1.2

31.1+/-0.3

anionic

anionic

CTS50

GQ334367

 β ++

 β +

27.5+/-0.1

27.2+/-0.1

anionic

anionic

CTS117

GQ334368

 β ++

 β ++

35.9+/-0.7

29.6+/-1.1

anionic

anionic

DR54

GQ334370

 β +

 β +

38.2+/-0.9

34.6+/-1.4

non ionic

non ionic

DSS73

GQ334363

α +

α +

32.1+/-0.9

33.2+/-0.1

anionic

anionic

DSS73-15C2

-

-

69.8 +/-0.9

70.8+/-0.3

non ionic

non ionic

CTS22

GQ334364

α +

α +

57.1+/-2.3

33.2+/-0.2

anionic

anionic

CTS70

GQ334365

α +

α +

31.4+/-0.1

30.1+/-0.0

anionic

anionic

CTS193

GQ334369

α +

α +

37.6+/-1.4

35.2+/-0.1

anionic

anionic

Table 1: Registration number of sequenced 16S rRNA fragments and surfactant production evaluated by hemolytic phenotype, surface tension and ionic charge of the bacterial rinsing solution for the 11 strains. Hemolysis on plate at 8°C and 17°C was realized as described in materials and methods. α corresponds to opaque lysis areas and β to transparent lysis areas. - : absence of clearing zone; + : lysis area≤1mm; ++: lysis area > 1mm. Surface tension of bacterial rinsing was determined as indicated in materials and methods at either 8°C or 17°C after four days (triplicate). The ionic charge determination was made with the rinsing solution of bacteria grown at either 8°C or 17°C as described in materials and methods.