Strains

Number of HPLC peaks

Molar mass (g.mol-1)

Retention time (min)

Growth at 8°C

Growth at 17°C

Growth at 8°C

Growth at 17°C

PfA7B

2

2

1111.7

24.0+/-0.7

24.2+/-0.0

1125.7

28.5+/-0.9

28.7+/-0.0

Pf1 Vis-

0

0

ND

ND

ND

DSS73

1

1

1394.8

16.0+/-0.6

16.0+/-0.6

DSS73-15C2

0

0

ND

ND

ND

CTS22

1

1

1353.8

16.2+/-0.6

16.0+/-0.5

CTS38

2

4

1111.6

ND

22.2+/-0.5

1125.7

25.1 +/-0.6

26.2+/-0.7

1151.6

ND

34.8+/-0.4

1153.7

36.9+/-0.5

38.0+/-0.0

CTS50

3

5

1111.6

22.1+/-0.8

22.0+/-0.5

1125.6

26.2+/-1.5

26.5+/-0.4

1139.6

31.0+/-1.0

31.0+/-1.0

1165.4

ND

38.0+/-0,9

1167.6

ND

41.1+/-1.1

CTS70

2

2

1394.8

15.7 +/- 0.3

15.7+/-0.3

1408.5

16.8 +/-0.5

16.8+/-0.5

CTS117

2

2

1111.7

21.2+/-0.6

21.1+/-1.0

1125.7

24.9+/-0.9

24.5+/-0.8

CTS193

1

1

1408.9

13.8+/-0.8

13.8+/-0.4

DR54

6

6

1110.7

45.5 +/-0.7

45.4+/-0.6

1124.7

46.9+/-0.4

46.9+/-0.4

1110.7

47.3+/-0.5

47.3+/-0.4

1124.7

48.7+/-0.4

48.8+/-0.4

1150.5

53.7+/-0.5

53.7+/-0.3

1152.6

57.0 +/- 0.5

56.9+/-0.3

Table 2: Biosurfactant characterization by HPLC for the nine wild strains grown at 8°C and 17°C HPLC was done for biosurfactants produced by the wild strains as described in materials and methods. ND means not detected. The experiments were done in triplicate.