Figure 3: Agarose gel electrophoresis of PCR products of cholera toxin gene (ctxA) and Acanthamoeba 18S rDNA gene. M are molecular mass markers (1500 bp), lane 1 amoebic positive control (approximately 450 bp), 2 bacterial positive control (308bp), 3 negative control, 4 to 6 samples contain both Acanthamoeba (a) and V. cholerae (b), 7 to 9 samples contain amoebae only, 10 sample containing V. cholerae only.