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Figure 6: Gram-determination und flow cytometric susceptibility testing from positive blood cultures. Gram determination (A) with SYTO® 9 and Hexidium iodide of S. hominis 1, uptake of Hexidium iodide led to red fluorescence (left) and a determination as Gram-positive. E. coli, showed green and red fluorescence due to the uptake of SYTO® 9 and Hexidium iodide (right) from blood culture and was determined as Gram-negative. Susceptibility testing (B) of S. hominis 1, red line: untreated control; filled grey histograms: samples with oxacillin, piperacillin/tazobactam, cefazolin, cefuroxime and gentamicin. |