Figure 3: SDS-PAGE of bean protein hydrolysates after different time intervals of trypsin hydrolysis (a-navy, b-black, and c-small red); and in vitro gastrointestinal simulation (GIS) digestion hydrolysates (e-navy, f-black, and g-small red) under reducing conditions (5% β‑ME): lane 1: 0 min; lane 2: 3 min after α-amylase digestion; lanes 3 to 8: 0 min, 5 min, 10 min, 30 min, 60 min, 120 min of peptic digestion, respectively; lanes 9 to 14: 0 min, 5 min, 10 min, 30 min, 60 min, 150 min of trypsin/α- chymotrypsin digestion, respectively.