Variable Coef. SE Coef. t-value P-valuea constant 45.5071 0.03842 1184.423 0.000 X1 0.9559 0.03622 26.389 0.000 X2 0.4127 0.03622 11.398 0.000 X3 0.3448 0.03622 9.519 0.000 X22 -0.5954 0.06404 -9.299 0.000 X32 -0.6129 0.06404 -9.572 0.000 X1X2 0.0931 0.04050 2.299 0.042 X1X3 -0.6706 0.04050 -16.559 0.000 X2X3 0.8356 0.04050 20.633 0.000 aSignificant at P<0.05 S = 0.1146 R2 = 99.5% R2(adj) = 99.1%

Table 3: Coefficients and t-values of process variables for mycoprotein production using face centered composite design.