Strains or plasmids Function Source

E. coli Top10

plasmid amplification

our laboratory

E. coli ET12567(pUZ8002)

plasmid demethylation

John Innes Center, UK

Industrial S. rimosus (SRI)

OTC producer

Shanxi Datong Antibiotic Company

pMD19T pMD19T-otrA pMD19T-otrB

gene amplification gene amplification gene amplification

TARAKA, Japan this study this study

pSET152

gene integration

pSET152-otrA pSET152-otrB

gene integration gene integration

this study this study

Table 1: Strains and plasmids used in this present study.