Parameters Without alcohol With alcohol
AE (Unit/mL) 0.11±0.01 0.48±0.01
TA (Unit) 0.54±0.05 2.39±0.05
Cp (μg/mL) 17.60±5.48 31.87±1.31
SA (Unit/mg) 6.08±0.19 14.98±0.49
PF 12.70±0.39 31.27±1.02
RY (%) 22.62±2.23 100.85±2.23
Forward extraction: AOT concentration 20 mM, pH7.0 Backward extraction: KCl concentration 1.5 M, pH7.5 *Sample size: 5 mL
Table 7: Effect of alcohol addition on chymotrypsin purification parameters.