Figure 5: Representative images of selected cells in agarose microchambers. (a) Phase-contrast image of arrangement of the selected smooth cells on agarose microchamber at one by one manner using micropipette (same image of Figure 3a). (b) Phase-contrast image of selected cells after 2 days cultivation. White arrowhead shows a shrinked cell. White arrow shows the microchamber in which the cell was disappeared during cultivation caused by apoptosis or inufficient attachment. (c) Phase-contrast image superimposed on the fluorescence image of the selected cells (2 days cultivation) after fixation with 4% Formaldehyde solution. Nucleus (Hoechst33342; blue), heavy chain cardiac myosin (red). White double arrowheads show the no-stained cells by cardiac myosin (i.e., non cardiomyocytes). (d) Phase-contrast image of arrangement of the selected rough cells on agarose microchamber at one by one manner using micropipette (same image of Figure 4a). (e) Phase-contrast image of selected cells after 2 days cultivation. White arrowhead shows shrinkage cell. (f) Phase-contrast image superimposed on the fluorescence image of the selected cells (2 days cultivation) after fixation with 4% Formaldehyde solution. Nucleus (Hoechst33342; blue), heavy chain cardiac myosin (red). Bars, 100 μm.