Procedure |
Operation |
SUT |
PT |
ST |
P-1/ SFR-101(300 ml Inoculation) |
CHARGE-Nutrient 1 |
- |
5.00 min |
Beginning of batch |
|
CHARGE-Water 1 |
- |
3.00 min |
After Nutrient 1 charge |
|
AGITATE-1 |
- |
5.00 min |
After Water 1 charge |
|
CHARGE-E. coli 1 |
- |
3.00 min |
After Agitation |
|
FERMENT-1 |
- |
5.00 hrs |
After E. coli 1 charge |
|
TRANSFER-OUT- E. coli |
- |
3.00 min |
After Fermentation |
P-2/ V-101(3 L Fermentation) |
CHARGE-Nutrient 2 |
- |
5.00 min |
After 5 hours of batch operation |
|
CHARGE-Water 2 |
- |
3.00 min |
After Nutrient 2 charge |
|
AGITATE-1 |
- |
30.00 min |
After Water 2 charge |
|
TRANSFER-IN- E. coli |
- |
Master-Slave with P-1 Transfer Out of E. coli |
Starts with Transfer in from P-1 |
|
FERMENT-1 |
- |
4.00 hours |
After Transfer in of E. coli from P-1 |
|
TRANSFER-OUT- E. coli |
- |
3.00 min |
After Fermentation |
|
CIP-1 |
- |
15.00 min |
After Transfer out to P-3 |
P-3 /V-102(Media Blending Tank) |
CHARGE-Nutrient |
0 min |
Calculated based on 600 L/h |
After 5.31 hours of batch operation |
|
CHARGE-Water |
20 min |
Calculated based on 600 L/h |
After nutrient charge |
|
AGITATE-1 |
- |
10.00 min |
After water charge |
|
TRANSFER-OUT-Media to P-5 |
20 min |
Calculated based on 600 L/h |
After Agitation |
|
STORE-1 |
- |
4.66 hours |
After Transfer Out of Media to P-5 |
|
TRANSFER-OUT-Media to P-7 |
20 min |
Calculated based on 600 L/h |
After Store |
|
CIP-1 |
- |
15.00 min |
After Transfer Out of Media to P-7 |
P-4/ ST-101(Heat Sterilization) |
STERILIZE-1 |
- |
15.00 min |
Starts with Transfer Out of Media (to P-5) in P-3 |
P-5/ V-103(30 L Fermentation) |
TRANSFER-IN-Media to P-5 |
20 min |
Calculated based on 600 L/h volumetric flowrate |
After Sterilize-1 in P-4 |
|
TRANSFER-IN-3 L E. coli |
- |
Master Slave with P-2 Transfer Out of E. coli |
Starts with Transfer Out of E. coli in P-2 (to P-5) |
|
FERMENT-1 |
- |
4.00 hrs |
After Transfer In of E. coli |
|
TRANSFER-OUT- E. coli |
20 min |
Calculated based on 600 L/h |
After Fermentation |
|
CIP-1 |
- |
45.00min |
After Transfer Out of E. coli |
P-6/ ST-101(Heat Sterilization) |
STERILIZE-1 |
- |
15.00 min |
Starts with Transfer Out of Media (to P-7) in P-3 |
P-7/ V-104(300 L Fermentation) |
TRANSFER-IN-Media P-7 |
20 min |
Calculated based on 600 L/h |
After Sterilize-1 in P-6 |
|
TRANSFER-IN-30 L E. coli |
- |
Master Slave with P-5 Transfer Out of E. coli |
Starts with Transfer Out of E. coli in P-5 (to P-7) |
|
FERMENT-1 |
- |
4.00 hours |
After Transfer In E. coli |
|
TRANSFER-OUT-Broth |
- |
Master Slave with P-8 Centrifuge |
After Fermentation |
|
CIP-1 |
- |
45.00min |
After Transfer Out Broth |
P-8/CF-101(Centrifugation) |
CENTRIFUGE-8 |
- |
20.00 min |
Starts with Transfer Out Broth in P-7 (to P-8) |
|
CIP-8 |
- |
10.00 min |
After Centrifuge |
P-9/V-104(Blending / Storage PBS) |
TRANSFER-IN-Pellet |
- |
Master Slave with P-8 Centrifuge |
Starts with Transfer Out Pellet in P-8 (to P-9) |
|
CHARGE-PBS |
- |
3.00 min |
After Transfer in Pellet |
|
AGITATE-9 |
- |
10.00 min |
After charge PBS |
|
TRANSFER-OUT-PBS+Pellet |
- |
15.00 min |
After agitation |
|
CIP-9 |
- |
15.00 min |
After Transfer Out of PBS+Pellet |
P-10/HG-101(Homogenization) |
HOMOGENIZE-10 |
- |
30.00 min |
Starts with Transfer Out of PBS+Pellet in P-9 (to P-10) |
