Gibco medium Custom medium 1. High-glucose Dulbecco’s Modified Eagle Medium (DMEM) -80%  •  Contains high glucose,  non-essential amino acids, sodium pyruvate, phenol red 2. Bovine Serum and FBS -10% 3. DMSO -10% 1. RPMI-1640 (12-702F)- 27.5%  •  Contains sodium bicarbonate (NaHCO3) 26.7 ml/L of 7.5% NaHCO3and 10.3  ml of 200 mM L-glutamine 2. FBS - 62.5% 3. DMSO -10%

Table 1: Formulations of the Gibco recovery medium and the custom medium used for cryopreservation of PBMC for 7 days at -65°C and -80°C.