Figure 5: Amplification of FII(G20210A) using primers for mutant A alleles (lanes 1,3,5) and normal G allele (lanes 2,4,6) showing positive bands (340bp) in lane 1, 3, 4, 6 with negative bands in lanes 2 , 5 indicating A/A homozygous mutant genotype in case 1 (lanes 1,2) and G/A heterozygous genotype in case 2 (lanes 3,4), G/G normal genotype in case 3 (lanes 5,6). M indicates molecular marker, Bands of size 250 bps belongs to F IX used as internal controls.