Figure 3: In vitro antigen specific T cell proliferation and cytokine production. 1x104 FBS+ or FBS- BM-DC pulsed with 1040-55 mimepitope peptide were used to stimulate 1x105 BDC2.5 CD4+ splenocytes in both FBS+ and FBSculture media for 96 hours. A. Tritiated thymidine uptake was measured during the final 18 hours of incubation. B-D. Supernatant was removed after 72 hours of incubation and the indicated cytokines were measured with a multiplex cytokine immunoassay.