| EXON |
Sense primer |
Antisense primer |
Annealing Tm |
| EXON1 |
5’-TCATCAGCCATTTGTCCTTTCA-3’ |
5’- GGAAGTGGGGATAGGAAAATACA -3’ |
58.4 (DMSO5%) |
| EXON2 |
5’-TCATGAACAGAAGAACGTCATT-3’ |
5’-CACAGTCATTTCAGGTAAGAA -3’ |
58.9 |
| EXON3 |
5’-GAAACTCCGCCTCAATAA -3’ |
5’-GAGACGGGATTTCACCAG-3’ |
51.2 |
| EXON4 |
5’- CTAAAGGGCAGTCTGTCAGT -3’ |
5’- TACTATTGAGGCTGGGTGTAT -3’ |
58.0 |
| EXON5 |
5’-TGGCTTTCATGCTATTCTGC-3’ |
5’-CCCCTTTTCATCACTTTCTTAT-3’ |
51.2 |
| EXON6 |
5’-TGTAAGCAACAGAGAGCTGT-3’ |
5’-ACCACACCTGGCCTCAGTT-3’ |
58.0 |
| EXON7 |
5’TGTTTAAATTCCCCTTTCCATC--3’ |
5’-TTTGAACCCACATGAGTGTCA-3’ |
58.0 |
| EXON8 |
5’–GAGTTGAAATTCCGATTTCTCT3’ |
5’-ACACCTGGCCAGTAGATGTT-3’ |
58.0 |
| EXON9 |
5’-TTAATGACATGGTTAGGGTCAA-3’ |
5’-GTTAGGTACTATAGGTAGAGAA-3’ |
56.0 |
| EXON10 |
5’TGGTATCAGAAATACCCCTGT-3’ |
5’-ATTTGTGGCATTAGGCAACTC-3’ |
58.0 |
| EXON11 |
5’-ATGGTTTGACATGTACTTTGTC-3’ |
5’-ATCTTGCACTTGTACCAAACAA-3’ |
58.0 |
| EXON12-1 |
5’-TGTACGTTTGGAAGAAAATG -3’ |
5’-ATTGTATTCATCTTGGCAACTC -3’ |
51.2 |
| EXON12-2 |
5’-GCAACTGGACAGCAGGACTT-3’ |
5’-AATAGTTATTTAAATGGCCCCA-3’ |
51.2 |
| EXON13 |
5’-AGTTACATCCCTTACCCGTTA-3’ |
5’-CACTCCATAGGCTTGAAAACA-3’ |
58.0 |
