Before furosemide and before intragastric medication Medication at 15 min before furosemide Time after furosemide (post-furosemide time) (min) 95 100 110 120 130 140 150 Time after medication application (post-therapy time) (min) Amplitudes of ECG P, R, S, T waves (0.01mV); P wave duration, QRS, QTintervals duration (msec) 110 115 125 135 145 155 165 P wave amplitude (0.01mV) 2.8± 1.0 CONTROL(saline 5ml/kg i.g.) 3.0± 0.6 3.1± 1.3 3.2± 0.9 3.3± 1.0 3.3± 1.4 3.4± 1.1 3.4± 1.6 2.8± 1.4 BPC 10μg/kg i.g. 2.7± 1.0 2.8± 1.7 2.9± 1.1 2.9± 1.2 2.9± 1.1 2.9± 1.9 3.1± 1.3 2.7± 0.9 BPC 10ng/kg i.g. 2.8± 0.4 2.9± 1.4 2.9± 0.9 2.9± 0.7 2.9± 1.6 2.9± 1.8 3.0± 1.8 R wave amplitude (0.01mV) 57.2± 1.9 CONTROL(saline 5ml/kg i.g.) 38.9± 2.7 37.9± 3.1 37.7± 2.4 35.2± 3.2 35.1± 2.7 34.9± 2.5 34.4± 2.1 58.4± 1.6 BPC 10μg/kg i.g. 52.9± 2.4 † 53.2± 2.3 † 52.2± 1.8 † 51.9± 2.4 † 52.2± 1.9 † 52.9± 2.7 † 51.6± 2.7 † 57.9± 1.5 BPC 10ng/kg i.g. 52.8± 2.7 † 54.4± 2.1 † 53.5± 1.9 † 53.7± 1.7 † 53.3± 1.6 † 53.8± 2.3 † 52.6± 2.1 † S wave amplitude (0.01mV) 22.6± 1.5 CONTROL(saline 5ml/kg i.g.) 13.5± 1.8 13.7± 2.1 13.5± 1.2 12.2± 2.4 12.8± 2.4 12.0± 2.7 12.2± 2.1 22.1± 1.2 BPC 10μg/kg i.g. 22.7± 1.2 † 23.1± 1.1 † 22.6± 1.6 † 22.7± 1.3 † 23.1± 1.2 † 23.0± 1.2 † 23.0± 0.9 † 22.5± 1.6 BPC 10ng/kg i.g. 22.1± 1.4 † 23.8± 1.3 † 22.9± 1.7 † 22.6± 1.4 † 23.5± 1.3 † 23.4± 1.6 † 23.1± 1.3 † T wave amplitude (0.01mV) 2.2± 0.9 CONTROL(saline 5ml/kg i.g.) 1.5± 0.8 1.5± 1.1 1.4± 1.2 1.4± 1.3 1.4± 1.5 1.4± 1.1 1.4± 0.9 2.1± 0.7 BPC 10μg/kg i.g. 2.4± 1.1 † 2.3± 1.2 † 2.3± 0.9 † 2.5± 1.2 † 2.5± 1.3 † 2.5± 1.1 † 2.6± 0.9 † 2.2±1.0 BPC 10ng/kg i.g. 2.2± 0.8 † 2.2± 1.3 † 2.3± 1.4 † 2.4± 1.6 † 2.3± 1.1 † 2.4± 1.5 † 2.4± 1.4 † P wave duration (msec) 17.2±1.1 CONTROL(saline 5ml/kg i.g.)¥ 25.3± 1.8 25.9± 1.4 26.3± 1.4 26.7± 1.8 26.9± 1.1 27.4± 2.1 27.9± 1.7 17.4±1.4 BPC 10μg/kg i.g¥ 22.4± 1.5 18.2± 1.1 † 17.4± 1.1 † 17.3± 1.7 † 17.1± 1.9 † 17.7± 1.6 † 18.0± 1.9 † 17.1±1.6 BPC 10ng/kg i.g.¥ 22.9± 1.4 19.0± 1.6 † 18.7± 2.1 † 18.4± 1.2 † 18.2± 1.4 † 18.7± 1.4 † 18.9± 2.3 † QRS interval duration (msec) 16.6±1.3 CONTROL(saline 5ml/kg i.g.)¥ 31.1± 2.1 31.9± 2.2 32.4± 2.6 32.7± 1.5 33.1± 1.5 33.8± 1.9 34.1± 1.8 16.7±1.2 BPC 10μg/kg i.g.¥ 29.9± 1.9 18.2± 1.7 † 18.0± 2.1 † 17.5± 1.7 † 17.3± 1.4 † 17.1± 1.5 † 17.2± 1.7 † 16.3±1.7 BPC 10ng/kg i.g. 30.1± 2.1 18.8± 1.7 † 18.5±1.9 † 18.1± 2.1 † 17.9± 1.2 † 17.7± 1.1 † 17.4± 1.1 † QT interval duration (msec) 81.6±2.1 CONTROL(saline 5ml/kg i.g.)¥ 104.2± 2.1 104.7± 1.3 104.9± 2.1 104.9± 2.9 105.3± 1.9 105.7± 2.1 105.8± 1.4 83.2±2.7 BPC 10μg/kg i.g.¥ 103.9± 2.7 85.3± 2.1 † 84.3± 1.9 † 83.2± 2.2 † 83.1± 1.7 † 83.4± 2.8 † 82.7± 1.7 † 83.9±2.4 BPC 10ng/kg i.g.¥ 104.4± 2.2 88.3± 2.5 † 87.7± 1.4 † 86.7± 2.7 † 85.4± 2.9 † 85.1± 2.1 † 83.5± 2.2 †

Table 4A: Effects of hypokalemia on amplitudes (0.01mV) of ECG waves, ECG wave duration and interval duration (msec). BPC 157 administered intragastrically was given prophylactically before furosemide (100 mg/kg intraperitoneally). Values are mean ± SEM. ¥ - Two-way ANOVA with repeated measure on one factor (P<0.05) difference between measurements depends on group membership.†P<0.05 significantly different from control values.