Primary Serum Clone Type Source Titer Antigen Retrieval Cyclin A   Monoclonal NEOMARKERS 1:200 CITRATE pH 6.0 Cyclin B1 V152 Monoclonal DAKO 1:1500 EDTA+TRIS pH 9.0 Cyclin D1 RBT-14 Monoclonal BIO-SB Pre-diluted EDTA+TRIS PH 9.0 p16 F12 Policlonal SANTA CRUZ 1:400 CITRATE pH 6.0 p21 SX118 Policlonal DAKO 1:50 CITRATE pH 6.0 p27 SX53G8 Monoclonal DAKO 1:200 CITRATE pH 6.0 p53 DO-7 Monoclonal DAKO 1:2000 CITRATE pH 6.0 Topoisomerase 2A SWT-3D1 Monoclonal DAKO 1:800 CITRATE pH 6.0 KI-67 MIB-1 Monoclonal DAKO 1:300 CITRATE pH 6.0 Rb NCL-RB358 Monoclonal NOVOCASTRA 1:300 CITRATE pH 6.0

Table 2: Primary serum, clones, source, titer and antigen retrieval.